Thinking and practice on establishing a high-quality university education system of laboratory medicine / 中华检验医学杂志

It is an urgent need for the development of medical and health undertakings in the new era to build a high-quality university education system and cultivate high-quality personnel for laboratory medicine. In the process of building a national first-class specialty, the medical laboratory technology specialty of Southern Medical University adheres to the concept of high-quality university education and improves the training level of laboratory medicine personnel through the teaching reform practice of constructing the whole-process moral education system, systematical training mode, internationalized teaching team, and intelligent teaching technology. In this paper, we analyzed the connotation and construction experience of a high-quality university education system of laboratory medicine, in order to increase the exchange and communication between different colleges and universities, and make contributions to the national strategic goal of building a powerful country in education before 2035.

Trend of mortality and decomposition on malignant tumors in Shandong province, 1970-2013 / 中华流行病学杂志

Objective@#To describe the mortality trend of major malignant tumors in Shandong province, from 1970 to 2013.@*Methods@#Data related to cancer mortality were obtained from the Shandong Death Registration System and three nationwide retrospective cause-of-death surveys. Trends of overall mortality and major causes of death were described using the indicators as: mortality rates and age-standardized mortality rates, through comparing the three large-scale mortality surveys in Shandong province. Difference decomposing method was applied to estimate the contribution of demographic and non-demographic factors for the change of mortality.@*Results@#From 1970 to 2013, the crude mortality rate of malignant tumors in Shandong was increasing. The age standard mortality rate was increasing and then decreasing. The composition of cancer deaths in the all-cause-deaths was seen increasing and then decreasing as well. Both demographic and non-demographic factors contributed to the increase of crude cancer mortality rate. With the gradual increase of the proportion of population, its role exceeded the non-demographic factors. The age-standardized mortality rate of malignant tumors in 2011-2013 was lower than that in 2004-2005. Lung cancer mortality rose from the fifth to the first place, with an increase of 6.81 times from 1970-1974 to 2011-2013. Ranking of gastric cancer mortality dropped from first to the third place, with esophageal cancer dropped from second to the fourth. After adjusted by China’s standard population in 1964, the mortality rate of lung cancer was still rapidly increasing, but the age-standardized mortality rates of esophageal cancer was gradually decreasing. The crude and age-standardized mortality rates of cervical cancer showed a rapid downward trend, reduced 87.00% and 93.00% respectively from 1970-1974 to 2011-2013.@*Conclusions@#Malignant tumors were still major threats to the residents of Shandong province. The changing trend of different malignant tumors presented an inconsistent nature which called for different intervention strategies be carried out, accordingly.

Evaluation on the effect of salt reduction intervention among fourth-grade primary school students and their parents in Shandong Province / 中华预防医学杂志

In order to evaluate the intervention effect of the school-based salt reduction model, 28 primary schools were selected in Shandong Province in September 2014 and randomly divided into intervention group (1 361 students, 1 306 parents) and control group (1 364 students,1 340 parents). A series of "small hands and big hands" salt reduction intervention activities were conducted in intervention group for 8 months. After the intervention, the total awareness rate of salt reduction knowledge, the total holding rate of related beliefs and the total reporting rate of related behaviors were 70.65%, 80.30% and 67.03% among students, and 85.66%, 93.77% and 87.93% among parents, in the intervention group, which were higher than those in the control group (37.12%, 66.52% and 50.07% among students; 55.11%, 87.52% and 57.96% among parents) (all P values <0.05). The school-based salt reduction model is effective and feasible.

Epidemiological characteristics and medical expenses of acute occupational poisoning hospitalization in 5 monitoring counties (cities, districts) of Shandong Province from 2012 to 2018 / 中华预防医学杂志

The data were drawn from injury hospitalization surveillance system in Shandong province. From 2012 to 2018, 164 cases of acute occupational poisoning were reported from five surveillance counties (cities, districts), accounting for 6.11% (164/2 683) of total accidental poisoning cases. The annual average reported incidence of acute occupational poisoning hospitalization was 1.15/100 000. The number of male cases was 3.3 times that of females (126 vs 38). The poisoning cases mainly occurred between January to May in a year and 5-7 AM within a day. Those cases were mainly caused by irritating gases (92 cases, 56.10%) and asphyxiating gases (53 cases, 32.32%), of which chlorine (71 cases) and carbon monoxide (50 cases) were the main reasons. The average hospitalization medical cost of acute occupational poisoning cases was 7 278.81 RMB per case.

Analysis of mortality and probability of premature death caused by four main non-communicable diseases in Shandong Province, 2015 / 中华预防医学杂志

Based on the data from Shandong Province death registration system in 2015, the mortality rate was adjusted by the results of underreporting rate investigation, and the probability of premature death of non-communicable diseases was calculated by life table method. A total of 728 287 deaths caused by four main non-communicable diseases (NCDs) including cardiovascular and cerebrovascular diseases, malignant tumor, diabetes, and chronic respiratory disease, accounting for 85.83% of the total death in 2015. The death of cardiovascular and cerebrovascular diseases and malignant tumor accounted for 50.90% and 25.69% respectively. The probability of premature death was 17.84%. The probability in males was higher than that in females (23.12% vs 12.25%). The highest probability of premature death in urban was cancer, but in rural was cardiovascular and cerebrovascular diseases. The probability increased from the East areas to the West. The highest probability reached at 21.50% in the Western underdeveloped areas, and the lowest probability was in Dongying City (14.84%) and Weihai City (14.94%) in the coastal areas.

Molecular phylogenetic studies on clinical bovine piroplasmosis caused by benign Theileria in Shaanxi Province, China

A group of benign Theileria species, which are often referred to as T. orientalis/T. buffeli/T. sergenti group, has low pathogenicity in cattle. Herein, we report on Theileria spp. in cattle on a farm from China. Based on phylogenetic analysis of the major piroplasm surface protein gene sequences, we detected 6 genotypes that were categorized as Types 1, 2, 3, 4, and 5 as well as an additional Type 9 genotype. The new epidemiological features of the T. orientalis/T. buffeli/T. sergenti parasites in China indicate a greater diversity in the genetics of these species than had been previously thought.

Research advances in the mouse model of primary biliary cholangitis / 临床肝胆病杂志

Primary biliary cholangitis (PBC) is an autoimmune disease characterized by the destruction of interlobular bile ducts and infiltration of inflammatory factors.An increase in anti-mitochondrial antibody is the major feature of PBC and may eventually lead to intrahepatic cholestasis,liver fibrosis,and even liver cirrhosis.The pathogenesis of this disease remains unclear,and there is no ideal treatment regimen.Therefore,it is necessary to find an animal model matching with PBC,in order to study the pathogenesis,treatment,and prognosis of PBC.This article introduces the research advances in bile duct ligation models,drug models,antigen models,gene models,and spontaneous models,in order to summarize the features and limitations of the mouse model of PBC in the articles published in recent years and provide a basis for the selection and research of PBC models.

Mercury accumulation of Tibetan medicine Zuotai in mice / 中成药

AIM To explore the mercury accumulation in KM mice after being given Zuotai at different doses and time.METHODS KM mice were randomly divided into blank group,Zuotai low-,middle-and high-dose (6.07,60.70 and 606.97 mg/kg,42 d;606.97 mg/kg,14 d) groups.The mercury contents in brain (olfactory bulb,cortex,hippocampus,hypothalamus,brain stem,cerebellum),heart,lung,kidney,liver,spleen,serum,muscle of mice were measured after administration.RESULTS Compared with the blank group,Zuotai at low-dose significantly increased the mercury contents in hippocampus,cerebellum,lung,kidney,liver and serum of mice after 42-day treatment;Zuotai at middle-dose markedly increased the mercury contents in olfactory bulb,cortex,hippocampus,brain stem,cerebellum,heart,lung,kidney,liver,spleen and serum of mice after 42-day treatment;the mice treated with high-dose of Zuotai for 42,14 days significantly increased the mercury contents in olfactory bulb,cortex,hippocampus,hypothalamus,brain stem,cerebellum,heart,lung,kidney,liver,spleen,muscle and serum.CONCLUSION Mercury can be accumulated in different tissues of mice after intragastric administration of Zuotai in a dose-and time-dependent manner,which suggests that Zuotai and its compound preparations should not be used in high-dose and long-term.

Prevalence of dyslipidemia among residents aged 18-69 years in Shandong province of China, 2011 / 中华预防医学杂志

<p><b>OBJECTIVE</b>To investigate the prevalence and distribution characteristics of dyslipidemia among Shandong residents aged 18-69 years.</p><p><b>METHODS</b>The levels of triglyceride (TG), low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C), and total cholesterol (TC) were determined in fasting serum of 5 ml venous blood for subjects aged 18-69 years who were selected by multi-stage stratified cluster random sampling from 20 counties in July, 2011 in 140 counties of Shandong province. The prevalence of dyslipidemia, hypercholesterolemia, high blood LDL-C, low blood HDL-C and hypertriglyceridemia were analyzed by the complex weighting. The prevalence of dyslipidemia, hypercholesterolemia, high blood LDL-C, low blood HDL-C and hypertriglyceridemia were compared for different characteristics by Rao-Scott χ(2).</p><p><b>RESULTS</b>The prevalence of dyslipidemia among 15 350 subjects was 22.70% (3 572), with higher in males (26.91% (2 110/7 683)) than in females (18.41% (1 462/7 667)). The prevalence of hypercholesterolemia was 3.39% (n=582), with higher in Eastern Shandong (4.59% (185/3 704)). The prevalence of high blood LDL-C was 0.56% (n=94), with higher in Eastern Shandong (0.79% (32/3 074)). The prevalence of low blood HDL-C was 11.41% (n=1 789). The prevalence of hypertriglyceridemia was 13.02% (n=2 059), higher in urban residents (16.54% (814/4 804)) than in rural (11.52% (1 245/10 546)) (χ(2)=71.54, P<0.001). The difference between the prevalence of low blood HDL-C and hypertriglyceridemia was not significant among Eastern Shandong, Central-South and Northwest.</p><p><b>CONCLUSION</b>Dyslipidemia rate was higher among adult residents in Shandong province. The rate was higher for men than for women. The prevalence of hypercholesterolemia and high blood LDL-C were higher in Eastern Shandong than the other areas. The hypertriglyceridemia and low blood HDL-C were two major types of dyslipidemia.</p>

Application of virtual simulation technology to laboratory medicine / 中华检验医学杂志

Medical virtual simulation teaching environment is an advanced and complex combination of virtual simulation technology and medical technology training .As an advanced information technology , it has been attracting the attention of medical education researchers .Based on the characteristics of medical laboratory technology education , the virtual simulation experimental teaching center was established and a framework of the virtual simulation training environment was designed in the Department of Laboratory Medicine, Southern Medical University .Virtual simulation softwares and tool softwares were put into use widely in our experimental teaching .As it turns out, this simulated training environment has many merits such as risk-free safety , great efficiency , genuine learning experience and wide application potential , which partly overcomes the restriction of the teaching process brought by the condition of the practical operation and improves the teaching effect significantly .

Association between 24 h urinary sodium to potassium ratio and metabolic syndrome in Chinese adults / 中华流行病学杂志

<p><b>OBJECTIVE</b>To examine the association between 24 h urinary sodium to potassium ratio and metabolic syndrome (MS) in Chinese adults.</p><p><b>METHODS</b>A population-based cross-sectional study was conducted among Chinese adults aged 18-69 years in Shandong province in 2011. Data on 24 h urinary excretion of sodium and potassium and components of MS were examined. Participants were divided into four groups according to the quartile of 24 h urinary sodium to potassium ratio.</p><p><b>RESULTS</b>Of the 1 906 Chinese adults eligible for final data analysis, 471 (24.7%) were with MS. After completion of multivariate logistic regression analysis, when compared to the participants with 24 h urinary sodium to potassium ratio < 4.3, the OR (95% CI) of participants with 24 h urinary sodium to potassium ratio during 4.3-5.6, 5.7-8.1, and ≥ 8.1 were 1.27 (0.93-1.71), 1.06 (0.78-1.46), and 1.45 (1.06-1.97), respectively (P values for linear trend < 0.05). As for the components of MS, the odds of central obesity and elevated blood pressure but not the odds of elevated triglycerides, low high density lipoprotein cholesterol and elevated fasting glucose, had significantly increases with successive 24 h urinary sodium to potassium ratio quartiles (P values for linear trends < 0.05).</p><p><b>CONCLUSION</b>The 24 h urinary sodium to potassium ratio appeared significantly associated with the odds of MS.</p>

Comparison of dietary survey, frequency and 24 hour urinary Na methods in evaluation of salt intake in the population / 中华预防医学杂志

<p><b>OBJECTIVE</b>To compare the difference and correlation between dietary salt intakes assessed by 24 hours urinary Na method, food weighted record method and food frequency questionnaire method.</p><p><b>METHODS</b>All 2 184 subjects aged 18 to 69 were selected by multi stage stratified cluster random sampling method in Shandong province in June to September, 2011. Dietary salt intakes were measured by 24 hours urinary Na method, food weighted record method and food frequency questionnaire method. The information on gender, age, dining locations and labour intensity of members dining at home for 3 days were recorded. And the dietary habits were surveyed by questionnaire.</p><p><b>RESULTS</b>Salt intakes were 14.0, 12.0 and 10.5 g/d assessed by 24 hours urinary Na method, food weighted record method and food frequency questionnaire, respectively. Comparing with 24 hours urinary Na method, salt intakes assessed by food weighted record method and food frequency questionnaire method were 2.0 g (14.3% undervalued) and 3.4 g (24.3% undervalued) less, respectively. Comparing with 24 hours urinary Na method, the proportion of individuals with salt intake over-reported and under-reported were 42.4% (856/2 020) and 55.3% (1 117/2 020) by food weighted record method, and were 20.7% (418/2 020) and 16.3% (329/2 020) by food frequency questionnaire method, respectively; the proportion of individuals with salt intakes within ± 25% of 24 hours urinary Na method were 36.9% (745/2 020) and 28.4% (574/2 020), respectively. Salt intakes assessed by 24 hours urinary method correlated significantly with both salt intakes assessed by food weighted record method and food frequency questionnaire method; the correlation coefficients were 0.13 and 0.07, respectively. With the increasing of salt intakes by subjects' self-judgment, salt intakes were all rising significantly using three survey methods. Salt intakes of three group population of light, moderate and partial taste salty were 13.6, 13.6 and 14.7 g/d by 24 hours urinary Na method (F = 0.47, P < 0.05); 11.0, 12.0 and 12.7 g/d by food weighted record method (F = 5.83, P < 0.05), and 9.3, 10.2 and 11.9 g/d by food frequency questionnaire method (F = 5.83, P < 0.05), respectively.</p><p><b>CONCLUSION</b>Comparing with 24 hours urinary method, food weighted record method and food frequency questionnaire method would undervalue dietary salt intake. Salt intake status can be more accurately assessed by adjusting the underestimation rate.</p>

Dietary sodium intakes and resources among residents in Shandong province / 中华预防医学杂志

<p><b>OBJECTIVE</b>To describe dietary sodium intakes and resources among residents in Shandong province.</p><p><b>METHODS</b>A total of 2184 subjects were selected by multi-stage stratified cluster random sampling method from 18-69 years old people in Shandong province in June, 2011. A total of 2140 subjects completed the study, the completion rate was 98.0%. Three-day (24-hour per day) dietary recalls and weighting methods were conducted to collect information about all the foods and condiments consumed by the subjects. Individual dietary sodium intake was calculated, the differences of dietary sodium intake among subjects with different characteristics were analyzed, and the proportions of different dietary sodium resources were also analyzed.</p><p><b>RESULTS</b>The amount of individual dietary sodium intake was 5745.0 (95%CI:5427.6-6062.5) mg/d in Shandong; 6147.4 (95%CI: 5823.8-6471.0) mg/d for male residents, 5339.3 (95%CI:5005.8-5672.8) mg/d for female residents. There was a significant difference between males and females (F = 75.22, P < 0.01). The amount of individual dietary sodium intake was 5910.1 (95%CI:5449.3-6370.8) mg/d, 5341.6 (95%CI:5007.0-5676.1) mg/d for rural residents and urban residents respectively, and there was also a significant difference (F = 5.53, P < 0.05). The amount of condiment sodium intake was 4640.3 (95%CI:4360.2-4920.4) mg/d, which was the largest contributor to sodium intake, accounting for 80.8% (95%CI:79.9%-81.6%) of total intake. Sodium intake from cereals was 650.7 (95%CI: 590.5-711.0) mg/d, accounting for 11.3% (95%CI:10.3%-12.3%) of total intake. Sodium intake from eggs was 118.9 (95%CI:95.2-142.6) mg/d, accounting for 2.1% (95%CI:1.6%-2.6%) of total intake. The amount of manufactured food sodium intake was 582.1(95%CI: 497.8-666.4) mg/d, accounting for 10.1% (95%CI:8.9%-11.4%) of total intake.</p><p><b>CONCLUSION</b>Sodium intakes remain high among residents of Shandong province, and sodium from condiments was the largest source of dietary sodium intake, sodium of manufactured food only accounting for small part.</p>

Analysis on prevalence states and associated factors of hypertension and prehypertension among adults in Shandong province / 中华预防医学杂志

<p><b>OBJECTIVE</b>To analyze the prevalence states of hypertension and prehypertension and to explore relevant influencing factors among adult residents in Shandong province.</p><p><b>METHODS</b>15 600 residents aged between 18 and 69 from 20 counties in Shandong province were selected by multiple stratified and clustered sampling method from July to September, in 2011, to acquire related information by questionnaire survey and physical measurement. The prevalence of hypertension and prehypertension was estimated by special statistic method used to deal with complex sampling data, and the relevant influencing factors were also analyzed.</p><p><b>RESULTS</b>A total of 15 350 subjects were actually completed the survey, with age at (41.4 ± 14.1) years old. The average systolic blood pressure and diastolic blood pressure were 121.1(95%CI: 119.7-122.4) and 78.8(95%CI: 77.8-79.9) mmHg (1 mmHg = 0.133 kPa) respectively. And also 3776 hypertension patients and 5721 subjects with prehypertension were detected, and the weighting prevalence of hypertension and prehypertension were 23.4% (95%CI: 20.9%-26.0%) and 37.1% (95%CI: 34.7%-39.5%) with adjustive prevalence at 20.7% and 36.5% respectively. The multiple SURVEYLOGISTIC analysis showed that age above 40 years old (OR = 3.24, 95%CI: 2.56-4.10), overweight(OR = 2.22, 95%CI: 1.70-2.89) and obesity(OR = 5.84, 95%CI: 3.54-9.66), smoking history(OR = 1.82, 95%CI: 1.03-3.23), constantly drinking (OR = 1.71, 95%CI: 1.08-2.70), diabetes (OR = 1.99, 95%CI: 1.29-3.07), abnormal TC(OR = 1.64, 95%CI: 1.24-2.17), abnormal TG(OR = 1.75, 95%CI: 1.24-2.48) and high 24 h urine Na/K ratio (OR = 1.05, 95%CI: 1.02-1.08) were risk factors of hypertension, while education equal to or above junior middle school (OR = 0.68, 95%CI: 0.52-0.89) was protective factor of hypertension; age above 40 years old (OR = 1.49, 95%CI: 1.15-1.91), overweight(OR = 1.76, 95%CI: 1.25-2.48) and obesity (OR = 3.50, 95%CI: 2.05-5.97), abnormal TC (OR = 1.54, 95%CI: 1.10-2.14), abnormal TG (OR = 1.79, 95%CI:1.25-2.56) and high 24 h urine Na/K ratio (OR = 1.02, 95%CI: 1.01-1.04) were risk factors of prehypertension, while female (OR = 0.41, 95%CI: 0.31-0.56) and education level equal to or above junior middle school (OR = 0.67, 95%CI: 0.52-0.87) were protective factors.</p><p><b>CONCLUSION</b>The hypertension, especially prehypertension tends to be at high prevalence states currently, which may be affected by many factors.</p>

The relationship between salt consumption and blood pressure among residents in Shandong province, China / 中华预防医学杂志

<p><b>OBJECTIVE</b>To study the relationship between salt consumption and blood pressure among residents in Shandong province, and to provide basic information for guiding people to prevent hypertension by reducing salt intake.</p><p><b>METHODS</b>Using multi-stage stratified cluster random sampling method, 2 184 residents aged 18 to 69 were selected as the research subjects, which were extracted randomly from 20 counties in Shandong province in June, 2011. The blood pressure of the subjects were measured and weighted. Based on 72 h dietary recalls and weighing measurement, and according to the distribution of salt intake, the grouping cut-off points were 10, 14 and 18 g. The difference of subjects with different characteristics and systolic blood pressure, diastolic blood pressure and prevalence of hypertension by grouping salt intake and other characteristics were analyzed, and the relationship between salt intake and prevalence of hypertension were analyzed.</p><p><b>RESULTS</b>The total of 2 133 residents were involved finally, the completion rate was 97.7%. After the complex weighted, the systolic and diastolic blood pressure's means (95%CI value) of the subjects were 121.0 (119.5-122.5) mmHg (1 mmHg = 0.133 kPa) and 78.5(77.4-79.6) mmHg respectively, which were all elevated with increasing of salt consumption. The blood pressure of the subjects with salt intake <10 g was the lowest, which were 119.6 (116.9-122.4) mmHg and 77.5(76.2-78.7) mmHg respectively. The blood pressure of the subjects with salt intake ≥ 18 g was the highest, which were 122.3(120.1-124.5) mmHg and 79.6(78.2-80.9) mmHg respectively. The analysis results showed that, among residents with normal waistline, the prevalence of hypertension of people with salt intake ≥ 18 g was 1.70(95%CI:1.04-2.76) times compared to that of people who consumed less than 10 g salt per day.</p><p><b>CONCLUSION</b>The hypertension prevalence of Shandong province is quite high, and there is a relationship between salt consumption and blood pressure.</p>

Construction and practice of the innovation talents training mode for laboratory medicine / 中华检验医学杂志

Based on the developing trend of laboratory medicine and the internal rules of discipline construction,series of research and reform practices in the curriculum system were put forward,including practice teaching,innovation education and instructional technology for laboratory medicine.An innovation talents training mode for laboratory medicine is gradually formed and innovative education is pushed on systematically,effectively and abidingly.The new mode has made periodical results and good prospects in cultivating students with innovative quality and ability.

Exploration and practice of the hospital-enterprise united cultivation pattern for laboratory medicine / 中华医学教育探索杂志

The Laboratory medicine is a practice subject.Department of Medical Laboratory in the first clinical medicine college(Nanfang Hospital),Southern Medical University strengthens communication and cooperation with enterprises,such as third parted laboratory medicine institutions and independent laboratories.The enterprises participate in teaching work including reforming curriculum system.cultivating students'practice and innovation abilities,constructing exercitation bases,sdvancing students'occupation competition,and so on.Along with emphasizing practice and innovation abilities cultivation,the hospital-enterprise cultivation pattern for Laboratory medicine was established and made primary progress.

Effect of TGF-β on collagen degradation of rabbit keratocytes / 吉林大学学报(医学版)

Objective:To observe the effect of TGF-β1 on collagen degradation of rabbit keratocytes.Methods:The activity of collagen degradation of keratocytes with three-dime nsional culture in collagen gel was determined with spectrophotometer.Results:TGF-β inhibited collagen degradation of keratocytes in dose-dependent manner.Conclusion:The present result suggests that TGF-β1 has the inhibitory effect on collagen degradation of keratocytes and may help in the treatment of corneal ulceration.